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You work for a biotechnology company and your project is to produce large quantities of the human protein Farabin in bacteria. What would be the correct order of steps for you to accomplish this?

Ligate the Farabin fragment into a plasmid vector with a lac promoter
Digest the Farabin gene and the vector with the same restriction enzyme
Add IPTG to antibiotic resistant cells
Derive a cDNA for the Farabin gene
Transform the ligation reaction into bacteria

I chose 2-4-1-5-3. Is this correct in my thinking process?

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