(Answered) You will be preparing a 500ml culture and purifying protein

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You will be preparing a 500ml culture and purifying protein ( lactate dehydrogenase)and each mutation. what is a complete procedure for this part of your project. Start with an agar plate with BL21(DE3) cells for each of your colonies. The mutation is changing the amino acid residue number 9 of the LDH monomer into a histidine.

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