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1. You require to prepare 200 uL of each protein at a concentration of 1mg/mL for an upcoming experiment. Calculate the recipe to make each desired solution at the above concentration and volume. Do you have enough materials to make each dilution? Note: Your diluent is water.

a. Alpha-tubulin: you have 500 uL at 5 mg/mL

b. TGFbeta: you have 300 uL at 2.4 mg/mL

c. Albumin: you have 150 uL at 1 mg/mL

d. ConA: you have 100 uL at 1.5 mg/mL

2. You have 1L of 10X SDS-PAGE Running Buffer and you need to make as much 1X running buffer as possible for the upcoming experiment. How much 1X running buffer can you make using the entire 1L of 10X running buffer?

 

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