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You need to calculate ligation for your insert. You know that you have 3 ng/μL in your sample, how many picomols/μL do you have if it is a 1000 bp insert?

Include your converstions and calculations.

Tips:A base pair has an approximate molecular mass of 660 g/mol*bp.

  • 109 ng = 1 g
  • 1012 pmol = 1 mol

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