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You have purified total RNA from leaf cells of a maple tree. You dissolved all the RNA in 50 ul (microliters) of pure water to make an "RNA Sample." You need to determine the concentration (ug/ul) of this RNA sample, to do this you removed 2 ul from the sample and added these to 998 ul of water and put in a cuvette. You determined the absorbance of light at 260nm for this 1ml of RNA water solution in the cuvette and the A260 value was 0.058. A260 of 1.0 = 40 ug/ml for RNA.

i) what is the concentration (ug/ul) of the RNA in the cuvette?

ii) what is the concentration (ug/ul) of the RNA in the original RNA sample?

iii) How many ul of the RNA sample would you need to use in an experiment that required a total of 10ug RNA?

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Category:- Biology
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