Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

You have isolated a new protein and are in the process of characterizing it. Several pieces of data about the protein are presented below. What can you deduce about the structure of this protein at primary, secondary, tertiary, and quaternary levels.

A) The protein elutes in a void volume on a gel filtration column with a fractionation range of 30kDa to 300kDa

B) SDS-PAGE(reduced) shows the protein has three bands with molecular weights of about 215, 18, and 22kDa.

C) Following brief treatment of the original protein with chymotrypsin, the resulting protein sample now elutes from the gel filtration column as two peaks (250, 130kDa)

D) The 250kDa sample from C) runs as a single band at 125kDa on SDS PAGE (reduced) SDS PAGE (reduced) of the other sample from C) shows it to contain 3 bands 90,22, and 18kDa

Biology, Academics

  • Category:- Biology
  • Reference No.:- M923999

Have any Question? 


Related Questions in Biology

Which phylloclade commonly found in xerophytic plants that

Which phylloclade commonly found in xerophytic plants that is modified?

Part aprepare a powerpoint slides meeting below grading

PART A Prepare a powerpoint slides meeting below grading criteria covering topic Isoflavones and Polymethoxyflavones using recent original articles one from cell, frontier and nature. -recent articles related to the mech ...

The plasma membranes of polarized epithelial cells such as

The plasma membranes of polarized epithelial cells, such as those found in thetubules of the nephrons in the kidney, are divided into apical and basolateraldomains. How are these two domains different and how do the diff ...

Is anolis cristatellus more closely related to anolis

Is Anolis cristatellus more closely related to Anolis occultus or Anolis evermanni?

Please help me with these questions1 describe how sex is

Please Help me with these questions: 1. Describe how sex is determined in humans 2. Describe what sex linkage is and give an example 3. Define dosage compensation. 4. Define nondisjunction and describe a scenario in whic ...

Select one of the topics research the topic thoroughly and

Select One of the Topics, Research the Topic Thoroughly and Write a Concise Research Paper Companion Animal Medicine Most of the concepts that apply to humans also apply to our companion animal friends, an important thin ...

What type of reproduction involves union of sex cells and

What type of reproduction involves union of sex cells and their nuclei?

Microevolution is in some ways random while in other ways

Microevolution is in some ways random, while in other ways it is not. Explain what does this mean in regards to the potential of a mutation saving a species facing extinction?

In this assignment you will select a fermented food or

In this assignment, you will select a fermented food or beverage from the list provided. After making your selection, you will prepare a 3 page written report about it. The report should include historical information re ...

Elisa uses an enzyme-linked antibody to detect and antigen

ELISA uses an enzyme-linked antibody to detect and antigen. Briefly explain how use of the enzyme is ELISA could amplify the detection of a very small amount of antigen in a sample.

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

WalMart Identification of theory and critical discussion

Drawing on the prescribed text and/or relevant academic literature, produce a paper which discusses the nature of group

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro