Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

You have isolated a new protein and are in the process of characterizing it. Several pieces of data about the protein are presented below. What can you deduce about the structure of this protein at primary, secondary, tertiary, and quaternary levels.

A) The protein elutes in a void volume on a gel filtration column with a fractionation range of 30kDa to 300kDa

B) SDS-PAGE(reduced) shows the protein has three bands with molecular weights of about 215, 18, and 22kDa.

C) Following brief treatment of the original protein with chymotrypsin, the resulting protein sample now elutes from the gel filtration column as two peaks (250, 130kDa)

D) The 250kDa sample from C) runs as a single band at 125kDa on SDS PAGE (reduced) SDS PAGE (reduced) of the other sample from C) shows it to contain 3 bands 90,22, and 18kDa

Biology, Academics

  • Category:- Biology
  • Reference No.:- M923999

Have any Question? 


Related Questions in Biology

Explain why the removal of great sharks poses a threat to

Explain why the removal of great sharks poses a threat to bay scallops on the Atlantic Coast of North America, despite the fact that large sharks do not feed on scallops. Include the concept of a trophic cascade in your ...

How many kidneys does a human being has when he is

How many kidneys does a human being has when he is born?

What are the transcription factors important for muscle

What are the transcription factors important for muscle development? i.e. There are different steps to get a muscle fiber, what are the transcription factors that are important for these steps?

Transgenic organismsthe development of transgenic organisms

Transgenic Organisms The development of transgenic organisms is an established technique in many laboratories. A transgenic organism is an organism that includes DNA from another organism in the cells of its body. This D ...

1 how does gel electrophoresis sort dna fragments2 if

1. How does gel electrophoresis sort DNA fragments? 2. If each individual has such a small amount of DNA, how do the bands on the gel contain enough DNA to be visible? 3. Many genes only have a few possible alleles. For ...

Please watch the attached videos after learning about both

Please watch the attached videos. After learning about both radiation therapy and hyperbaric medicine, select one and write a detailed research paper on that topic. Radiation therapy videos: https://www.youtube.com/watch ...

Given what you know about how natural selection works

Given what you know about how Natural Selection works, explain why Sickle cell disease hasn't been eradicated from the population.

Assignment1 what do pollen dust mites certain foods and

Assignment 1) What do pollen, dust mites, certain foods and drugs, insect venom, fungal spores, and some ingredients in cosmetics have in common? they often contain dangerous bacteria they often act as allergens they all ...

1 describe the difference between a tumor suppressor gene a

1. Describe the difference between a tumor suppressor gene, a proto-oncogene, and an oncogene. Provide an example of each. 2. Cervical cancer is mainly caused by human papilloma viruses (HPVs). Explain how viruses contri ...

Generally aneuploidies are caused by the impaired

Generally, aneuploidies are caused by the impaired assortment of chromosomes during meiosis. For example, they are caused when the homologous chromosomes of pair 21 do not separate and, therefore, gametes with two chromo ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen