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1. What is meant by the term "clone"?
Why is it important to obtain large quantities of a particular DNA sequence?
List the steps you would take to obtain a clone of a DNA sequence.

2. For each of the following, give a brief description:
restriction enzyme
vector
recombinant DNA molecule
sticky ends

3. What is the process of transformation?
What is meant by competent cells?
What would you use a selection system for?

4. What information could you obtain from a southern blot?
What information could you obtain from a northern blot?
Describe what a genomic library is.

5. You are a forensic scientist and you perform a southern analysis to try and link a possible suspect to the scene of a crime. Draw the results of such a southern analysis and decide if the suspect was present or not.
Describe a possible application for making a transgenic animal or plant.

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  • Category:- Biology
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