Ask Question, Ask an Expert

+61-413 786 465

info@mywordsolution.com

Ask Biology Expert

I want help with this transformation efficiency problem:

You add up 10 ng of pGEM to 50 ul of competent cells, and then dilute this into a total of 1 ml Luria broth after the transformation. You take 100 ul of the Luria broth and plate on and agar plate. The next day, there are 780 colonies on the plate. Determine the transformation efficiency?

Biology, Academics

  • Category:- Biology
  • Reference No.:- M937712

Have any Question?


Related Questions in Biology

Do polynucleotides make the proteins dna polymerase 1 dna

Do polynucleotides make the proteins DNA polymerase 1, DNA Polymerase 3, Helicase, SSB proteins, ligase and Primase?

The united states established the first national park in

The United States established the first National Park in the world on March 1, 1872. What does this say about the people of the United States?

If you dont water a plant it will eventually die explain

If you don't water a plant it will eventually die. Explain why the absence of water halts photosynthesis. Also, cells that use aerobic cellular respiration, like humans, do not produce ATP at normal levels without oxygen ...

What are the equations for molarity molality density ppm

What are the equations for Molarity, molality, density, ppm, ppb, mass percent, Volume percent, and mole percent?

Title titer mtl what do you mean when you refer to the

Title Titer MTL: what do you mean when you refer to the burst size of a phage? How will burst size affect your Titer?

A how are chromosomes positioned on the metaphase

a. How are chromosomes positioned on the metaphase plate in metaphase 1 of meiosis? b. Is this different from metaphase in mitosis? If so, how?

In a cross between two heterozygous aa individuals what is

In a cross between two heterozygous (Aa) individuals, what is the likely percentage of the offspring that will be heterozygous

Question write a 525- to 700-word paper on the genetic

Question: Write a 525- to 700-word paper on the genetic disorder (Sickle Cell Disease ). Include the following in your paper: Summarize the Chromosomal Theory of Inheritance and how chromosomal abnormalities can lead to ...

Which are the logical steps proposed by margulis that would

Which are the logical steps proposed by Margulis that would help explain why the accumulation of oxygen in the atmosphere led to the evolution of mitochondria.

I am having trouble identifying if my buffer solution is a

I am having trouble identifying if my buffer solution is a weak acid and salt....or weak base and salt. Often my questions just give me then names of two molecules and I do not understand how to differentiate. For exampl ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Why might a bank avoid the use of interest rate swaps even

Why might a bank avoid the use of interest rate swaps, even when the institution is exposed to significant interest rate

Describe the difference between zero coupon bonds and

Describe the difference between zero coupon bonds and coupon bonds. Under what conditions will a coupon bond sell at a p

Compute the present value of an annuity of 880 per year

Compute the present value of an annuity of $ 880 per year for 16 years, given a discount rate of 6 percent per annum. As

Compute the present value of an 1150 payment made in ten

Compute the present value of an $1,150 payment made in ten years when the discount rate is 12 percent. (Do not round int

Compute the present value of an annuity of 699 per year

Compute the present value of an annuity of $ 699 per year for 19 years, given a discount rate of 6 percent per annum. As