problem 1:

prepare down the principle features of the pUC cloning system.

problem 2:

Illustrate out the term DNA modifying enzymes. Provide ex.

problem 3:

Critically illustrate out the term expression vectors. Provide ex for mammalian expression vectors.

problem 4:

Provide the significance of fusion proteins. prepare down the vectors employed for the gene fusion.

problem 5:

prepare down the steps in the cloning of the DNA segments in plasmid vector by poly dA: poly dT tailing technique?

problem 6:

Make a distinction between pGEM 3Z and pBR327.

problem 7:

Delineate invitro packaging.

problem 8:

Illustrate out the term transfection.

problem 9:

How could you raise the activity of plant promoter.

problem 10:

prepare down the modifications which required converting a Ti plasmid in to a cloning vector.