What would be the resulting 6 base sequence after combining the XhoI-generated sticky end with a SalI-generated sticky end? (You can choose more than one correct answer.)
The restriction endonuclease, XhoI, recognizes the sequence 5′-CTCGAG-3′ and cleaves between the C and T on each strand.
The restriction endonuclease, SalI, recognizes the sequence 5′-GTCGAC-3′ and cleaves between the G and T on each strand.
| CTCGAG |
| CAGCTC |
| GTCGAC |
| GAGCTG |
| CTCGAC |
GTCGAG
|