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1) prepare down the enzymes that are involved in gene manipulation?

2) prepare a detailed note on purification of DNA.

3) What is the use of pBR 322 as a cloning vector? prepare a detailed note on its special feature.

4) Describe how do you alter lembda phage virus into the cloning vector?

5) describe yeast episomal plasmid in detail.

6) Describe Ti plasmids in detail.

7) describe Northern Blotting technique.

8) prepare a detailed note on chemical method of DNA delivery.

9) prepare down applications of DNA technology in medicine.

10) What are of the applications of gene cloning in agriculture?

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