While running an ELISA at work, you are chatting with a co-worker about your weekend plans. The conversation is marvelous and you seem to finish your work in no time. As you check your results, you realize the measured signal intensity (ie. color change in your 96-well microtiter plate) is much higher than you have ever seen, even in wells containing controls you have run several times before. You realize that your chatting must have caused you to skip a step in the protocol. What error is the likely source of this intense color and are your results still valid?