Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

1. Because G binds to C more strongly than A binds to T, the melting temperature should increase with the fraction of G/C pairs along the backbone of a sequence.

a. Plot the melting temperature as a function of G/C content for a 60 base pair sequence by varying the number of G/C pairs between 15 and 45 bases. Plot the melting temperature versus the fraction G/C content. Does the melting temperature depend linearly on the fraction of G/C pairs, and if so, what is the slope and the intercept? Make sure you show the sequences you used.

b. compare the melting temperature of the original strand (60 bases, 30 C/G bases) at different concentrations of salt, NaCl, up to physiological salt concentration (0.15 M NaCl).

2. To support 25 cycles of PCR in which you start with a single double strand where each strand is 4000 bases, what concentration of each nucleotide would you need to start with assuming each of the nucleotides is distributed throughout the DNA randomly? Assume 1 ml of solution is the total volume.

3. A 3700 bp sequence is digested with two separate restriction enzymes, KpnI and EcoR1. When digesting with KpnI, all EcoR1 sites are left in tact. Similarly, when digesting with EcoR1, all KpnI sites are left in tact. In the following, the results of two separate digestions are written. Assume each enzyme cuts with a staggered cut. What are the locations of the KpnI and EcoR1 sites on the 3700 bp original sequence?

KpnI digestion:
100 bp, no intact EcoR1 site, 5' staggered end, 3' staggered end.
500 bp sequence, with intact EcoR1 site 200bp from 5' end, 5' blunt end, 3' staggered end.
1400 bp sequence, 2 intact EcoR1 sites 200bp and 800 bp from 5' end, 5' staggered end, 3' staggered end.
500 bp, no intact EcoR1 site, 5' staggered end, 3' blunt end.
500 bp, intact EcoR1 site 300 bp from the 5' end, 5' staggered end, 3' staggered end.
700 bp sequence, no intact EcoR1 site, 5' staggered end, 3' staggered end.

EcoR1 digestion
600 bp sequence, no intact KpnI site, 5' staggered end, 3' staggered end.
1300 bp sequence, two intact KpnI sites, 300 bp and 1000 bp from the 5' end, 5' staggered end, 3' staggered end.
1100 bp, one intact KpnI site 600 bp from the 5' end, 5' staggered end, 3' blunt end.
200 bp, no intact KpnI site, 5' blunt end, 3' staggered end.
500 bp sequence, two intact KpnI sites, one 200 bp and one 300 bp from the 5' end, 5' staggered end, 3' staggered end.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M924290
  • Price:- $55

Guranteed 36 Hours Delivery, In Price:- $55

Have any Question? 


Related Questions in Biology

In this assignment you will select a fermented food or

In this assignment, you will select a fermented food or beverage from the list provided. After making your selection, you will prepare a 3 page written report about it. The report should include historical information re ...

Ocean systemsaccording to the law of conservation of energy

Ocean Systems According to the law of conservation of energy, energy cannot be created or destroyed, but it can change from one form to another 1. A boy doing a cannonball into the pool. He went from potential to kinetic ...

You read in the newspaper about a recently approved

You read in the newspaper about a recently approved prescription drug that has suddenly been withdrawn from the market because of severe adverse effects and several deaths. Discuss why this can still occur despite years ...

1 e coli is a shortened name for a bacterium that lives in

1. E coli is a shortened name for a bacterium that lives in our gut. According to the rules of binomial nomenclature it is properly written as _______________. a. Escherichia COLI b. Escherichia Coli c. Escherichia coli ...

As we get older we are prone to many conditions that affect

As we get older we are prone to many conditions that affect our bones, muscles and nerves. In your opinion how can these conditions be prevented?

Why was there such a diversity of species in such a small

Why was there such a diversity of species in such a small area? Could these species have been modified from an ancestral form that arrived on the Gal√°pagos Islands shortly after the islands were formed?

Use the following link Use the following link

Use the following link (http://www.ted.com/playlists/144/should_we_redesign_humans) to access a playlist of videos oncurrent developments in synthetic biology. Choose three videos to watch, and write a case study of at l ...

Discussion topicolder people die for a number of reasons

Discussion Topic Older people die for a number of reasons: cancer, heart failure, complications due to diabetes, stroke, Alzheimer's, etc. Often times, the way we live our lives affect not only how long we live but what ...

Please watch the attached videos after learning about both

Please watch the attached videos. After learning about both radiation therapy and hyperbaric medicine, select one and write a detailed research paper on that topic. Radiation therapy videos: https://www.youtube.com/watch ...

Outline the differences between a special sensory motor and

Outline the differences between a special sensory, motor and mixed cranial nerve, and briefly describe the functions of each sensory component of the cranial nerve What are EPSPs and IPSPs, and how are they produced? Exp ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen