+1-415-315-9853

info@mywordsolution.com

## Ask Biology Expert

 Algebra Math Calculus Physics Chemistry Biology Earth Science Physiology History Humanities English Sociology Nursing Science

1. Because G binds to C more strongly than A binds to T, the melting temperature should increase with the fraction of G/C pairs along the backbone of a sequence.

a. Plot the melting temperature as a function of G/C content for a 60 base pair sequence by varying the number of G/C pairs between 15 and 45 bases. Plot the melting temperature versus the fraction G/C content. Does the melting temperature depend linearly on the fraction of G/C pairs, and if so, what is the slope and the intercept? Make sure you show the sequences you used.

b. compare the melting temperature of the original strand (60 bases, 30 C/G bases) at different concentrations of salt, NaCl, up to physiological salt concentration (0.15 M NaCl).

2. To support 25 cycles of PCR in which you start with a single double strand where each strand is 4000 bases, what concentration of each nucleotide would you need to start with assuming each of the nucleotides is distributed throughout the DNA randomly? Assume 1 ml of solution is the total volume.

3. A 3700 bp sequence is digested with two separate restriction enzymes, KpnI and EcoR1. When digesting with KpnI, all EcoR1 sites are left in tact. Similarly, when digesting with EcoR1, all KpnI sites are left in tact. In the following, the results of two separate digestions are written. Assume each enzyme cuts with a staggered cut. What are the locations of the KpnI and EcoR1 sites on the 3700 bp original sequence?

KpnI digestion:
100 bp, no intact EcoR1 site, 5' staggered end, 3' staggered end.
500 bp sequence, with intact EcoR1 site 200bp from 5' end, 5' blunt end, 3' staggered end.
1400 bp sequence, 2 intact EcoR1 sites 200bp and 800 bp from 5' end, 5' staggered end, 3' staggered end.
500 bp, no intact EcoR1 site, 5' staggered end, 3' blunt end.
500 bp, intact EcoR1 site 300 bp from the 5' end, 5' staggered end, 3' staggered end.
700 bp sequence, no intact EcoR1 site, 5' staggered end, 3' staggered end.

EcoR1 digestion
600 bp sequence, no intact KpnI site, 5' staggered end, 3' staggered end.
1300 bp sequence, two intact KpnI sites, 300 bp and 1000 bp from the 5' end, 5' staggered end, 3' staggered end.
1100 bp, one intact KpnI site 600 bp from the 5' end, 5' staggered end, 3' blunt end.
200 bp, no intact KpnI site, 5' blunt end, 3' staggered end.
500 bp sequence, two intact KpnI sites, one 200 bp and one 300 bp from the 5' end, 5' staggered end, 3' staggered end.

• Category:- Biology
• Reference No.:- M924290
• Price:- \$55

Guranteed 36 Hours Delivery, In Price:- \$55

Have any Question?

## Related Questions in Biology

### Discussionmuch of health assessment planning evaluation and

Discussion Much of health assessment, planning, evaluation, and policy making depend on communication. How can the Shannon-Weaver communications model be applied to these? What noise (interruptions or barriers) could sta ...

### Mutations can lead to missing or malformed proteins that

Mutations can lead to missing or malformed proteins that can lead to disease. A group of scientists in the United Kingdom have published a paper in the American Journal of Human Genetics stating that the average person h ...

### Human biologydiscussion 1- identify and compare the two

Human Biology Discussion 1- Identify and compare the two major cardiovascular circuits in the human body. Include the purpose of each and the organs involved. Do not cut and paste information from the text or other sourc ...

### Introduction of disease provide a brief description of the

Introduction of disease. Provide a brief description of the disease/disorder. This part of the paper should not be limited to the definition of the disease.Include epidemiology as appropriate.Etiology Identify common cau ...

### Ethical decisions in the workplaceafter reading your

Ethical Decisions in the Workplace After reading your background readings, please review the following scenario and address the questions at the end. Jerry Stevens heads up an excavation crew for the municipal utilities ...

### Chose a topic and answer the questionsthe topic is

Chose a topic and answer the questions The topic is : Metabolic rate measures and methods Prompts: 1. What is the method? General information about the methods. 1. Why would a researcher use this method? The particular f ...

### Unit idiscuss why it is important that the public be

Unit I. Discuss why it is important that the public be knowledgeable about science and how it applies to the real world. Unit II Oil spills in the ocean are often treated with chemical dispersants. These materials are si ...

### Review scientific articlewrite one page bullet points

Review scientific article Write one page bullet points format about scientific article. Article: Desert hedgehog is a mammal-specific gene expressed during testicular and ovarian development in a marsupial by William A O ...

### Option i the effect of low ph on enzyme activitydesign an

Option I: The Effect of low pH on Enzyme Activity Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful ...

### Pick a type of cancer to research find articles that answer

Pick a type of cancer to research. Find articles that answer questions concerning your chosen type of cancer. An annotated bibliography consists of two parts: the reference citation in APA format, followed by the summary ...

• 4,153,160 Questions Asked
• 13,132 Experts
• 2,558,936 Questions Answered

## Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

### A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

### What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

### Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

### Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

### Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate