A) Using the data given in table below plot a Lineweaver-Burk plot

b) Determine the Km and Vmax for the three sets of data

c) Which inhibitor would the most effective at high substrate concentrations?

d) Explain you choice

Table 1 :

Kinetics data for an enzyme catalysed reaction with and without the presence of inhibitors :

[S] mmol/L : 1.67 - 2.50 - 5.00 - 10.00

Initial velocity of reaction (micromol/min) :

without inhibitior : 0.100 - 0.125 - 0.167 - 0.200

with inhibitor A : 0.083 - 0.106 - 0.149 - 0.185

with inhibitor B : 0.068 - 0.083 - 0.105 - 0.122