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In order to study transcriptional regulation and attenuation in the tryptophan operon, site-directed mutagenesis was used that resulted in the conversion of the UGG codons for tryptophan in the trp leader mRNA sequence into AUG codons. (3 pts) a) Under what conditions would the operon initiate transcription? b) Under what conditions would attenuation take place? c) Under what conditions would transcription continue through to the end of the operon?

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