1. Transfer of DNA from a donor to a recipient cell by a bacteriophage.
Transfer of DNA from a donor to a recipient as naked DNA in solution.
3. Feedback inhibition differs from repression because feedback inhibition
a. islessprecise.
b. is slower acting.
c. stops the action of preexisting enzymes. d. stops the synthesis of new enzymes.
e. all of the above
4. Bacteria can acquire antibiotic resistance by all of the following except
a. mutation.
b. insertion of transposons. c. conjugation.
d. snRNPs.
e. transformation.
5. Suppose you inoculate three flasks of minimal salts broth with
E. coli. Flask A contains glucose. Flask B contains glucose
and lactose. Flask C contains lactose. After a few hours of incubation, you test the flasks for the presence of ?-galactosidase. Which flask(s) do you predict will have this enzyme?
a.A d.AandB b. B e. BandC c. C
6. Plasmids differ from transposons in that plasmids a. becomeinsertedintochromosomes.
b. are self-replicated outside the chromosome.
c. move from chromosome to chromosome.
d. carry genes for antibiotic resistance.
e. none of the above
Use the following choices to answer questions 7 and 8.
catabolite repression b. DNA polymerase
c. induction
d. repression e. translation
page1image41008 page1image41168 page1image41328 page1image41488
7. Mechanism by which the presence of glucose inhibits the lac operon.
8. The mechanism by which lactose controls the lac operon.
9. Two offspring cells are most likely to inherit which one of the
following from the parent cell?
a. a change in a nucleotide in mRNA b. a change in a nucleotide in tRNA c. a change in a nucleotide in rRNA d. a change in a nucleotide in DNA e. a change in a protein
10. Which of the following is not a method of horizontal gene transfer? a. binaryfission
b. conjugation
c. integration of a transposon
d. transduction
e. transformation
Restriction enzymes were first discovered with the observation that
a. DNA is restricted to the nucleus.
b. phage DNA is destroyed in a host cell.
c. foreign DNA is kept out of a cell.
d. foreign DNA is restricted to the cytoplasm. e. all of the above
The DNA probe, 3? -GGCTTA, will hybridize with which of the following?
a. 5?-CCGUUA b. 5?-CCGAAT c. 5? -GGCTTA d. 3?-CCGAAT e. 3? -GGCAAU
The following enzymes are used to make cDNA. What is the second enzyme used to make cDNA?
a. reverse transcriptase c. RNA polymerase
b. ribozyme d. DNA polymerase
If you put a gene in a virus, the next step in genetic modification would be
a. insertion of a plasmid.
b. transformation.
c. transduction.
You have a small gene that you want replicated by PCR. You add radioactively labeled nucleotides to the PCR thermal cycler. After three replication cycles, what percentage of the DNA single strands are radioactively labeled?
a. 0% d. 87.5%
b. 12.5% e. 100%
c. 50%
Match the following choices to the statements in questions 7-10
antisense, southern blot, vector, clone, library

Pieces of human DNA stored in yeast cells.
A population of cells carrying a desired plasmid.
Self-replicating DNA for transmitting a gene from one organism
to another.
A gene that hybridizes with mRNA.

3. which of the following is the fourth basic step to genetically modify a cell?
a. transformation b. ligation c. plasmid clevage d. restriction-enzyme digestion of gene e. isolation of gene