1. You are making a solution to stain cells. The solution has to have a concentration of 10ug/ml Coomaisse blue in water. If you are making 0.5L, how much Coomaisse blue do you need?
2. To stain your cells, you need to apply 0.75mL of staining solution to the fixed cells. How much is this in uL?
3. When you make a slide, you only want a single layer of cells fixed so that you can see everything clearly. In the culture you are working with, the cells are at a concentration of 10^6 cell/mL. You only want 100 cells on a slide, how many uL of your culture do you need? Can you measure this volume using a pipette?