Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

1. To express a mammalian gene in bacteria, should the promoter be of bacterial origin or mammalian origin and why?

2. List 3 features that are essential for a plasmid to function as a cloning vector?

3. Given a 1kb gene cloned into a plasmid vector, what are two ways by which you can generate large amounts of this gene?

4. If you want to identify genes from human liver that are highly expressed only under conditions of high stress, what kind of DNA library will be the best one to use and why? How will you construct this library?

5. Why do Type II Restriction Endonucleases not degrade bacterial chromosomal DNA?

6. If a double-stranded circular DNA molecule has 4 recognition sites for HindIII, how many fragments will be generated if you digest that molecule with HindIII?How many fragments will result if you linearize the above ds DNA molecule and then digest with HindIII? describe!

7. If you are tasked with screening a cDNA expression library for the production of a specific protein, what kind of library screening method will you use and why?

1. A biotech company has obtained tissue from normal mice and from mice that has especially engineered, highly active muscle cells. You are hired by the company to identify the genes (mRNA) that are differentially expressed in the normal versus the engineered mice. describe in one page how you will design an experiment to complete your assignment. Your response should specifically address the problem.

2. Suppose you have successfully isolated the putative gene that confers resistance to viral flu, but now you need to show that the gene does actually protect you from getting the flu. Briefly, describe two different ways by which you can do the functional characterization of the putative viral flu gene that you have isolated, in a mouse model?

3. Let's say you have two bacterial samples in tubes 1 and 2, one of which has been transformed with ONLY a vector and the other with a RECOMBINANT construct. Using ONLY two techniques-PCR and gel electrophoresis (use both), how will you determine which tube carries a recombinant and which tube the vector alone? Describe where the primers will anneal (not specific sequence but general location), what you expect to observe on the gel and how you will analyze and draw conclusions from the results? A schematic may be helpful 

Biology, Academics

  • Category:- Biology
  • Reference No.:- M944287

Have any Question? 


Related Questions in Biology

John has difficulty swallowing loss of taste sensation in

John has difficulty swallowing, loss of taste sensation in the posterior one-third of the right half of his tongue, and decreased salivation, especially on the right side. Describe the sensory portion of the affected neu ...

Assignmentshort answeressay write your answers and submit

Assignment Short Answer ESSAY. Write your answers and submit assignment via the attachment. Please be specific and use examples from the textbook when appropriate 1) What 4 factors do you think should be taken into consi ...

Why was there such a diversity of species in such a small

Why was there such a diversity of species in such a small area? Could these species have been modified from an ancestral form that arrived on the Galápagos Islands shortly after the islands were formed?

1 how cando you reduce the amount of waste and pollution

1. How can/do YOU reduce the amount of waste and pollution that you create?  In other words, describe 3 things that you can do/already do to reduce, reuse and recycle more. 2. The City of Phoenix recently started a progr ...

Compare the path of an amino acid to that of a large fatty

Compare the path of an amino acid to that of a large fatty acid from absorption to delivery to a cell.

For training and development classcompare and contrast two

(For Training and Development Class) Compare and contrast two learning theories. Which one do you believe is most effective? Why? Your response should be at least 200 words in length.

Concerning a patient with autonomic dysreflexiawith what

Concerning a patient with autonomic dysreflexia: With what you know of the etiology for the hypertension, was labetalol a good choice for managing the blood pressure? Why or why not? If not, what other kind of drug do yo ...

Which part of the human brain is the centre of memory

Which part of the human brain is the centre of memory, learning, thinking and reasoning?

How does complementary base pairing facilitate dna

How does complementary base pairing facilitate DNA replication and the processes of transcription and translation? Identify the base pairs in each process.

Dolly dolly and more dollyit all started with a sheep named

Dolly, Dolly, and More Dolly It all started with a sheep named Dolly. In the mid-1990s, scientists proved convincingly that after decades of trying, we could, indeed, clone mammals - and even possibly, human beings. Unsu ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

WalMart Identification of theory and critical discussion

Drawing on the prescribed text and/or relevant academic literature, produce a paper which discusses the nature of group

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro