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These are questions from Western Blotting Lab. Please answer any question you know!

1)Why do proteins travel through the gel to the cathode?
2)What might a given lane look like upon Coomasie staining?
3)Which migrates farther, smaller protein or larger proteins?
4)Draw a schematic of the transfer "sandwich".
5)What are the qualities that of PVDF membrane that make it ideal for blotting?
6)How do the proteins get transferred from the gel to the membrane?
7)What is the importance of blocking step?
8)Draw a schematic of an antibody molecule and lable its antigen binding sites and it conserved sites.
9)What are primary antibodies specific for?
10)What are secondary antibodies specific for?
11) How are antibodies produced?
12) How does the detection method work?

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Category:- Biology
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