Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

A 2500 base-pair long cDNA of unknown sequence was cloned into the MCS of a plasmid vector that has universal priming sequences adjacent to and on both sides of the MCS. On one side this is called the "forward universal priming sequence" and on the other it is called the "reverse universal priming sequence." Sanger sequencing was done using primers that are specific for each of these universal priming sequences and approximately 700 nucleotides of sequence was determined from each primer. Assuming it is not possible to obtain sequence analyses longer than 700 nucleotides, describe the steps that must be done to obtain the remainder of the sequence of the cloned cDNA.

There are two very different methods that will accomplish this. One method requires much more effort and is less reliable than the other. Either method will receive full credit.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M924778

Have any Question? 


Related Questions in Biology

1 what are the benefits of sequencing the genome of an

1) What are the benefits of sequencing the genome of an organism? Explain by giving 3 examplesfor the applications of genomic information. 2) Why do not modern apes become human today? World limit is between 250-400

Focusing on thenbsprespiratory digestive urinary and

Focusing on the  respiratory, digestive, urinary, and reproductive systems.   Create a scenario  and give your patient a disease associated with any of these systems and present the history and symptoms of the particular ...

Are there some bacteria that are not easily stained by the

Are there some bacteria that are not easily stained by the gram stain? Can you give an example of a bacterium that might not show up as gram-positive or gram-negative after being stained by Gram's method?

What are the steps in the process of creating a safe

What are the steps in the process of creating a safe schools/health student initiative?

Problem 1 the schlogl modelthis model is famous for its

Problem 1: The Schlogl Model This model is famous for its bistable steady-state distribution. The reactions are              c1 B1 + 2X ? 3X,              c2      c3 B2 ? X,      c4 where B 1 and B 2 denote buffered spec ...

Microbiology1 for what specific purpose was gram staining

Microbiology 1. For what specific purpose was gram staining developed for? what is it used for today? 2. Why is the presence of air bubbles a sign of contamination? 3. Why is a gram stain helpful when monitoring bacteria ...

What percentage of the dna changes that occur and one cell

What percentage of the DNA changes that occur and one cell in one year escape the proofreading and repair process calculate this in both cases: if one mutation accumulates in one sale per year and if five mutations accum ...

Due to the prospect of interplanetary travel how would you

Due to the prospect of interplanetary travel, how would you attempt to  Isolate  and  identify  a bacterial infection acquired on another planet. This bacteria is unknown to use and does not appear in  Bergey's  or the  ...

Once the hypothalamus has detected low levels of calcium in

Once the hypothalamus has detected low levels of calcium in the bloodstream, describe the full cascade of events that leads to low bone density. Also describe the cascade that allows the dairy product to contribute to ci ...

Blame for commercial overexploitation can be placed on the

Blame for commercial overexploitation can be placed on the people who directly do the exploiting and those that buy the product - but which group deserves more of the the blame? Why? What is an example of a commercial re ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Section onea in an atwood machine suppose two objects of

SECTION ONE (a) In an Atwood Machine, suppose two objects of unequal mass are hung vertically over a frictionless

Part 1you work in hr for a company that operates a factory

Part 1: You work in HR for a company that operates a factory manufacturing fiberglass. There are several hundred empl

Details on advanced accounting paperthis paper is intended

DETAILS ON ADVANCED ACCOUNTING PAPER This paper is intended for students to apply the theoretical knowledge around ac

Create a provider database and related reports and queries

Create a provider database and related reports and queries to capture contact information for potential PC component pro

Describe what you learned about the impact of economic

Describe what you learned about the impact of economic, social, and demographic trends affecting the US labor environmen