There are two documents for lab procedures. You should start with "Rat CV Part 1", and then move on to "Soda Lime and Rebreathing".
Before you start:
• Make sure you have all viewe the surgery video, it is helpful to have it cued up on the computer to walk through the surgery.
• Before beginning, or during the course of your surgery, before you start treatments you should jointly fill out another CV data table that summarizes what you as a group expect to observe during the treatments.
• Take a deep breath. Surgery is not easy. It is easy to get frustrated and then make mistakes. Go slow, be careful, focus, and be smart.
What you need to turn in:
Report #3 - Rodent Cardiovascular Physiology (100pts)
You will again be working with your lab group to turn in a single lab report. All documents that are part of your report must be turned into your TA in both hard copy and electronic format (unless otherwise instructed by your TA).
Your report for this set of experiments does not need to be in traditional lab report format. You need to include the following sections (please label them accordingly in the document you turn in):
I. Pre-experiment:
A. Include a copy of your jointly created hypothesized CV data table results.
Title this table "Hypothesized Results" (5 pts)
II. Data/Results: (40pts total)
A. Figures showing example traces of treatment effects. Treatments include
all rows labeled on the CV table plus rebreathing with soda lime, rebreathing, and lactic acid injection treatment. Your figures should include traces for all treatments that show an effect. (20pts)
B. A filled out CV data table reflecting your actual results. (5pts)
C. Create another data table with the same column headings but label rows:
rebreathing with soda lime, rebreathing, and lactic acid injection
treatments. (5pts)
D. Write a brief summary of your results (what are the figures showing us,
how clear or unclear were responses....) (10pts)

Discussion: (27pts)
A. Discuss sympathetic and parasympathetic control of cardiovascular function. This discussion should include:
• What is the difference between cephalic and caudal stimulation of the vagus nerve.
• How the drugs used led to the observed physiological response.
• An explanation of the rebreathing and rebreathing with soda lime methodology. You need to explain how these rebreathing methodologies led to the observed physiology.
• What is lactic acid and how does it affect CV activity?
B. Note any differences between your hypothesized results, your observed results, and known physiology. Use literature resources to explain any discrepancies, providing at least one explanation for observed results.
III. Further thought discussion questions: (15pts)
• The protocol has you stimulate the right vagus nerve. What is the difference between stimulation of the right vagus versus the left vagus? How are they connected to the heart? What would you expect to see upon left vagus stimulation?
• Suggest at least one additional treatment or related experiment you could do with this preparation. What would you do, what would it test, what might you expect to happen?
III. References: (10pts)
• You may use textbooks and review articles as citations for the discussion and further thought questions, but you MUST include at least TWO original research articles.
• Citations must be correctly embedded in the text (Smith et al., 1999) and as a list at the end, in standard reference format.
The remaining 3pts are for overall clarity of presentation.
The report will be graded based on the quality of your data, the presentation of your results, the thoroughness and appropriateness of your discussion, and the completeness of your references.