The uv absorption in proteins at 280 nm is largely due to, Ask an Expert

Academics

The UV absorption in proteins at 280 nm is largely due to tryptophan residues. The molar extinction coefficient of tryptophan is 5380 M-1cm-1. Exactly 0.25 g of a protein is dissolved in 200 milliliters of a neutral buffer. The molecular mass of the protein is 36,000 g mol-1. The absorbance of the solution at 280 nm in a 0.5 cm thick cuvette is 0.37. From this data, calculate the number of tryptophan residues that are found in one protein molecule.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M9368422

Have any Question?Write your Review or question?

Related Questions in Biology

A how are chromosomes positioned on the metaphase

a. How are chromosomes positioned on the metaphase plate in metaphase 1 of meiosis? b. Is this different from metaphase in mitosis? If so, how?

A doctor is testing the effectiveness of a new antibiotic

A doctor is testing the effectiveness of a new antibiotic. He gives the first group of patients a placebo, a second group receives antibiotic A while the third group receives antibiotic B. Which of the groups is consider ...

If someone has been sitting without standing for 6 hours

If someone has been sitting without standing for 6 hours, and blood has been pooling in their veins, why would they feel dizzy when they stood up? How does this related to stroke volume and mean arterial pressure?

Two true-breeding stocks of pea plants are crossed one

Two true-breeding stocks of pea plants are crossed. One parents has red, axial flowers and the other has white terminal flowers; all F1 individuals have red, axial flowers. The genes for flower color and location assort ...

Igfbp2 rbp4 and factor d post bariatric surgeryigfbp2 what

IGFBP2/ RBP4 and Factor D Post Bariatric Surgery IGFBP2 ( what the normal physiological action in the body? And how it affectedby obesity? andpost bariatric surgery?) RBP4 (what the normal physiological action in the bod ...

If you diluted 1 ml of a sample into 9 ml of water then

If you diluted 1 ml of a sample into 9 ml of water, then took 1 ml of that and diluted into another 9 ml of water, and did that another two times, what is fold the dilution factor?

Bio toxicology lab assignment -based on the available

Bio Toxicology Lab Assignment - Based on the available information provided to you, lab work and lab reports, answer the following questions. 1) Potentially harmful chemicals are introduced into our environment every day ...

Is this statement correct or incorrect whymuscle tension in

Is this statement correct or incorrect? Why? Muscle tension in cardiac contractile muscle can be increased by temporal summation.

Question summaryfor readability please be sure to

Question: Summary For readability, please be sure to double-space your assignments. A new wild strain of nopal cactus has been identified as having remarkable promise to solve the international dietary manganese deficit ...

Why soap changes the surface tension of waterand why adding

Why soap changes the surface tension of water? And why adding water into penny, the average of water drops in 90oC temperature water is less than average of water drops in room temperature

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert