The recipe for 10x tbe buffer is 890 mm tris 890 mm boric, Ask an Expert

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1. The recipe for 10x TBE buffer is 890 mM Tris, 890 mM boric acid, 20 mM EDTA. What is the concentration of 1x TBE buffer?
2. A recipe calls for 10 ml of a 25mM stock of EDTA. However, all you have on hand is a 500 mM stock. How much more concentrated is what you have relative to what the recipe calls for? (i.e. what is the relative concentration).
3. The protocol for transforming Chlamydomonas requires 300 ul of a culture at a density of 1x108 cells/ml. The culture you have is at 1x106/ml. What is the concentration of your culture relative to what the protocol calls for?

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