As a new graduate student in a translational genetics research lab at Emory University in Georgia, you have selected a thesis project which requires you to develop three assays for a set of newly described mutations in a gene associated with a familial drug and alcohol addiction disease. The combination test will allow the tailoring of therapy for persons susceptible to addictive behavior. A patient has only one of the mutations. The only instruments available to you are only a PCR instrument, gel electrophoresis equipment, and DNA sequencing instrument.

Describe, in technical detail, a method (only one for each example) you would use to identify the mutation.