1. A change in the primary sequence of a protein will never affect allosteric regulation.

a) True b) False

2. Which of the following is not a mechanism of enzyme regulation?

a) feedback inhibition by a product

b) addition or removal of phosphate groups to amino acids with non-polar sidechains.

c) feedforward stimulation by a product.

d) proteolytic cleavage

e) none of the above - all are examples of actual mechanisms

3)Which of these is NOT true about the active site of an enzyme?

a) it is composed of distinct amino acids that perform catalytic activities

b) it can have a prosthetic group depending on the enzyme

c) it is formed by the folding of the protein

d) it is the site of binding of a non-competitive inhibitor

e) none of the above

4) The Km represents the substrate concentration that will cause an enzyme to be at half maximal velocity.

a) true b)false

5) Which is not true about Vmax:

a) is the maximal velocity at which an enzyme can function

b) is reduced by a non-competitive inhibtor

c) is determined on a Lineweaver-Burke plot by taking the reciprocal of the y-intercept

d) is low for an enzyme with a low Km and a high substrate affinity

e) none of the above - all are true