The KM of a Michaelis-Menten enzyme for a substrate is 1.0 x 10-4 M. At a substrate concentration of 0.2 M, νo = 43 μM x min-1 for a certain enzyme concentration. However, with a substrate concentration of 0.02 M, νo has the same value.
(A) Using numerical calculations show that this observation is accurate.
(B) What is the best range of [S] for measuring KM?