The kinetics analyses of an enzyme are measured as a, Ask an Expert

Academics

The kinetics analyses of an enzyme are measured as a function of substrate concentration in the presence and in the absence of 2mM inhibitor (I).

[S] (µM): 3, 5, 10, 30, 90

Velocity (µmol/min) No Inhibitor: 10.4, 14.5, 22.5, 33.8, 40.5

Velocity (µmol/min) With Inhibitor: 4.1, 6.4, 11.3, 22.6, 33.8

(a) What are the values of Vmax and Km in the absence of inhibitor? In its presence?
(b) What type of inhibition is it?
(c) What is the Ki of the inhibitor?
(d) Is [S] = 10µM and [I] = 2mM, what fraction of the enzyme molecules have a bound substrate? A bound inhibitor?

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M9368142

Have any Question?Write your Review or question?

Related Questions in Biology

The human microbiome and its relationship to human health

The human microbiome and its relationship to human health, what aspect to the human microbiome is vital?

Discussion board 3 dnaafter reading the prompt below post

Discussion Board 3: DNA After reading the prompt below, post your answer using about 200 words. Post your initial response at least 24 hours prior to the assignment due date, then return to respond to another classmate's ...

Instructions address each question below as it relates to

Instructions: Address each question below as it relates to the caw study given. A patient was brought to the Emergency Department by ambulance with two arrow wounds. One arrow is still in the patient on the left side; en ...

A mother has a blood the child has type o mr white has type

A mother has A blood, the child has type O, Mr. White has type B blood, and Mr. Green has AB. Whose the father? Mr. White or Mr. Green; Either Mr. White or Mr. Green; Neither Mr. White or Mr. Green could be the father an ...

Igfbp2 rbp4 and factor d post bariatric surgeryigfbp2 what

IGFBP2/ RBP4 and Factor D Post Bariatric Surgery IGFBP2 ( what the normal physiological action in the body? And how it affectedby obesity? andpost bariatric surgery?) RBP4 (what the normal physiological action in the bod ...

Question phytoplankton chemosynthesis and mitochondriafor

Question: "Phytoplankton, Chemosynthesis, and Mitochondria" For your primary post, please respond to one of the following three topics with a post of at least 125 words that addresses each point given in the instructions ...

If someone has been sitting without standing for 6 hours

If someone has been sitting without standing for 6 hours, and blood has been pooling in their veins, why would they feel dizzy when they stood up? How does this related to stroke volume and mean arterial pressure?

Question - gene cloning a please write the steps to clone

Question - Gene Cloning a) Please write the steps to clone the protease gene from Bacillus strain whose genome sequence is not known. b) Express the protease gene to obtain the enzyme in high yield, please plan your prot ...

Question darwin was not the first to consider evolution as

Question: Darwin was not the first to consider evolution as a process but he did come up with the first effective explanation for how it happens. Describe Darwin's theory of evolution by natural selection. Explain how th ...

Question this is the final project of the case

Question: This is the final project of the case study 1. Identify the specific case study you chose and the tool(s) you would employ to analyze the healthcare environment. 2. Provide an explanation of how the selected to ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert