The information to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by
CO2 (aq) + H2O (l) -->H+ (aq) + HCO3- (aq)
|
[CO2] (mmol * L^-1)
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R (�µmol * L^-1 * s^-1)
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1.25
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28.74
|
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2.50
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48.61
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5.00
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80.28
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20.00
|
155.59
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Use these values to determine the value of KM, the Michaelis-Menten constant. Answer the four parts below.
a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the corresponding blank.
[CO2] (mmol * L^-1) graph as R (�µmol * L^-1 * s^-1) graph as
2.50 --> ________ 48.61 --> ________
5.00 --> ________ 80.28 --> ________
b) Calculate the slope using the data points from part (a). Then determine the y-intercept using the second point.
Slope= _______ s y-intercept= ___________ L * s * mmol^-1
c) Use the y-intercept to calculate Rmax.
Rmax=___________ mmol * L^-1 * s^-1
d) Use Rmax and the slope to calculate Km.
Km=___________ mmol * L^-1