The following statements describe SDS-PAGE, a method used to separate proteins. Some of the statements are correct, and some of them are incorrect. Select all the correct statements.
Statements:
-SDS-PAGE uses a polyacrylamide gel to separate proteins.
-Sodium dodecyl sulfate denatures the proteins, resulting in protein separation by charge only.
-Smaller proteins migrate farther than larger proteins
-In SDS-PAGE, the more negatively charged proteins elute first from the column.
-Sodium dodecyl sulfate dentures proteins, resulting in proteins that are the same shape. (Assume beta-mercaptoethanol is also present to reduce disulfide bonds.)
-In SDS-PAGE, proteins migrate toward the negative electrode.