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The galactose represser protein from E. coli has a pI of around 5.9. While purification protocols were being designed, and it was found to bind to a Mono-S column at pH values of 7 and below. (Mono-S columns encompass S-type sulphonic acid groups attached to the resin and are strong cation exchangers.)

What is unusual regarding this observation, and describe these results?

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  • Category:- Biology
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