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The following experiments to characterize the organization of the rutabaga genome and obtain the following results.
Experiment 1: You prepare a genomic library that consists of 1 kb fragments of the rutabaga genome. You pick 100 clones at random, isolate the 1 kb genomic DNA fragments from these clones, label the fragments with radioactivity, and hybridize each fragment individually with a gel blot of rutabaga genomic DNA. In this experiment, you find that 60 clones yield hybridization pattern A and 40 clones display hybridization pattern B.
On the basis of this experiment alone, what can you conclude about the organization of repeated and single copy DNA in the rutabaga genome?

Experiment 2: You next prepare a genomic library consisting of 10 kb fragments from the rutabaga genome. You pick 1,000 clones at random, isolate the 10 kb genomic DNA fragments from these clones, label the fragments with radioactivity, and hybridize each fragment individually with a gel blot of rutabaga genomic DNA. You find that 800 clones give DNA gel blot hybridization pattern A and the other 200 clones give hybridization pattern B.
Based on the results of these two sets of experiments, what can you conclude about the organization of single copy and repeated DNAs in the rutabaga genome? Do the results indicate whether the rutabaga genome is organized in a short period or long period interspersion pattern? 

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  • Category:- Biology
  • Reference No.:- M993968

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