Ask Question, Ask an Expert

+1-415-315-9853

info@mywordsolution.com

Ask Biology Expert

All living organisms must utilize energy to maintain themselves and to carry out life activities. The rate of energy utilization is known as the metabolic rate. Eukaryotic organisms used chemical energy to drive energy requiring life processes. We will measure metabolic rate in both an anaerobic and aerobic organism by measuring the amount of C02 gas produced by these organisms. We will use the Vernier CO2 gas sensor inserted into an environmental chamber in which the organism is fermenting or respiring.

The oxidation of glucose to form carbon dioxide and water liberate energy through the process of photosynthesis. In photosynthesis, green plants convert sugars into chemical energy with the help of the sun. The sugars provide stable form in which energy can be stored and transported. However, these energy is converted into another chemical form known as adenosine triphosphate (ATP). When ATP is split, energy is release for cellular work and the resulting molecule is known as adenosine diphosphate (ADP). ADP is reconverted to ATP with energy derived from sugars or other nutrients.

The flow of energy from solar radiation captured by plants (via photosynthesis) to its use by both plants and animals. This energy drives many fundamental life processes such as movement, growth, and reproduction. The release of energy from food molecules occurs by two biochemical pathways. The first is called fermentation. Fermentation usually occurs in the absence of oxygen and referred to being anaerobic. The other biochemical mechanism of energy release is called cellular respiration.

Respiration ( Plants and animals)

O2 + sugar ---------------------------> CO2 + H2O + ATP ( chemical potential energy)

Photosynthesis ( plants and plant like protists)

CO2 + H2O + light energy -------------------------> O2 + sugar

Fermentation

C6H12O6 + 2Pi + 2 ADP ------> 2 CH3CH2OH + 2 CO2 + 2ATP + heat
Glucose Ethanol

The equation shows that for every molecule of glucose consumed: two moles each of ethanol, carbon dioxide, and ATP are produced as well as heat. You will begin by measuring the rate of CO2 productoin by yeast undergoing alcoholic fermentation. This rate will be related to the rate of ATP production which is a reflection of metabolic rate of yeasts.

The experiment will address the following problems:
Fermentation

problem 1: How will increasing the number of yeast cells influence the metabolic rate of the yeast suspensions?

problem 2: How will substituting galactose for glucose influence the metabolic rate of the yeast suspensions ?

Preparation of Yeast Samples:

1. Set up the three fermentation samples in test tubes according to the values specified in the following table:

Tube Label Suspending solution: Weight Yeast (grams)
Volume, % age, and type
1 glu- 0.3 6 ml - 20%- glucose 0.3
2 glu - 0.6 6ml - 20% -glucose 0.6
3 gal - 0.6 6ml - 20%-galactose 0.6

Measure gas production by your samples. Measurement can proceed when the sugar/yeast mixture begins to bubble and the parafilm bulges upward.

1. Turn on Lab Quest recorder by pressing the silver button on the upper left hand side of the unit.
2. Pour the entire yeast mixture from the test tube into petri dish and place the dish inside the large environmental chamber. Attach the cover which should have one opening. Insert CO2 sensor intot he second opening.

problem 3

describe why measurements of an organism's rate of gas consumption or production can be used to estimate that organism's rate of ATP production.

problem 4

What is the comparison of the two quantities of yeast exposed to the same concentration of glucose (tube 1 and tube 2)?

problem 5

describe the results for the comparison of the trials run with 0.6 grams yeast and either 20% glucose and 20% galactose.

Cellular Respiration by Corn Seedlings

Cellular respiration is the main process of ATP productoin used by plants, animals, and many microorganisms. The use of oxygen in the conversion of sugars to CO2 and water releases much more energy stored in sugar than fermentation does. It is about 16 times as much ATP is generated from fermentation of the same sugar. The overall reaction is shown below
C6H12O6 + 6 O2 + 32 ADP + 36Pi -------> 6CO2 + 6 H20 + 32 ATP + heat
The measurement of the rate of CO2 production by aerobic organism provides a useful measure of its rate of energy production. We will use a Vernier CO2 gas sensor inserted into a closed system containing corn seedlings undergoing cellular respiration.
The data will be used to address the problem

problem 6:
In terms of the mass specific rates you find outd (g ATP/ gram of tissue x hour), did the yeast suspensions or corn seedlings have a higher metabolic rate?

Preparatoin of corn seedlings:
1. Obtain and weigh a 250 ml Nalgen bottle and record its weight. Tilt the bottle on its side and add about 25 corn seedlings with radicles, ranging from 1.0 to 3.0 cm in length.
2. Once the seeds are in the bottle, weigh the bottle with seeds. The weight of seeds can then be find outd and recorded on worksheet.
Measurement of CO2 production

1. Insert CO2 sensor into the neck of Nalgene bottle.
2. Measure the CO2.

Biology, Academics

  • Category:- Biology
  • Reference No.:- M924398

Have any Question? 


Related Questions in Biology

1 there are two events that define a sexual life cycle

1. There are two events that define a sexual life cycle: formation of haploid gametes (egg and sperm) via Meiosis and fusion of gametes (fertilization) to form a diploid embryo. List and then describe two different proce ...

An irregular shaped machine component is held in the

An irregular shaped machine component is held in the position shown by three clamps. Knowing that FA = 940N, determine the magnitudes of the forces FB and FC exerted by the other two clamps.

Real world lab-use what you have learned about microscopes

Real World Lab- Use what you have learned about microscopes to- 1. View and sketch the Letter "e" slide at all three levels of magnification- - be sure to label your sketches according to total magnification - take an im ...

Assignmentsimmunology1st assignmentsection ithe following

Assignments Immunology 1st Assignment Section I. The following Topics 1-3 (of 4) cover the cell isolation protocols from Units 1 & 2: Assigned Appendix readings; Isolation of human monocytes by double gradient centrifuga ...

1 while working on snake blenny biochemistry you discover a

1. While working on snake blenny biochemistry, you discover a collection of five new molecules (a, b, c, d, e) shown below. The color of each molecule represents a distinct sugar. You decide to look for mutants to define ...

Option i the effect of low ph on enzyme activitydesign an

Option I: The Effect of low pH on Enzyme Activity Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful ...

Recall that the biological species concept defines a

Recall that the biological species concept defines a species as a group of populations whose members have the potential to interbreed in nature and produce viable, fertile offspring. This means that all members of these ...

Biologygo to httpwwwecfrgovwrite again in essayparagraph

Biology Go to: http://www.ecfr.gov/ Write again in essay/paragraph format with the question content incorporated into the sentences and not written separately. 1- Go to Title 40. What is the title of CFR 40? 2- Go to par ...

Dna and rnacompare and contrast dna and rna discuss why

DNA and RNA Compare and contrast DNA and RNA. Discuss why humans did not evolve with one central repository of DNA, but rather it is replicated throughout the body? Your assignment should be 250-500 words in length.

Answer in a word or sentence1 what is decibel name any one

Answer in a word or sentence: 1. What is decibel? Name any one source that can produce noise of about 120 decibel. 2. What are the effects of smog on plants? 3. Define Pollutants with any one example. 4. What is acid rai ...

  • 4,153,160 Questions Asked
  • 13,132 Experts
  • 2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

A cola-dispensing machine is set to dispense 9 ounces of

A cola-dispensing machine is set to dispense 9 ounces of cola per cup, with a standard deviation of 1.0 ounce. The manuf

What is marketingbullwhat is marketing think back to your

What is Marketing? • "What is marketing"? Think back to your impressions before you started this class versus how you

Question -your client david smith runs a small it

QUESTION - Your client, David Smith runs a small IT consulting business specialising in computer software and techno

Inspection of a random sample of 22 aircraft showed that 15

Inspection of a random sample of 22 aircraft showed that 15 needed repairs to fix a wiring problem that might compromise

Effective hrmquestionhow can an effective hrm system help

Effective HRM Question How can an effective HRM system help facilitate the achievement of an organization's strate