The absorbance a of a solution is defined as alog 10 ioi in, Ask an Expert

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The absorbance A of a solution is defined as A=log 10 (Io/I) in which Io is the incident light intensity and I is the transmitted light intensity. The absorbance is related to the molar absorption coefficent e (in M-1 cm-1), concentration c (in M) and path length (in cm) by A=elc. The absorbtion coefficient of myoglobin at 580 nm is 15,000 M-1 cm-1.

a) what is the absorbance of a 1mg ml-1 solution across a 1-cm pathlength?

b) what percentage of the incident light is transmitted by this solution?

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