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Suppose your lab instructor asked you to make quantitative determinations of the following:

  1. Total protein concentration in whole blood.
  2. Concentration of a solution of purified hemoglobin.
  3. Concentration of hemoglobin in whole blood.

Would you expect the BCA assay to give accurate values for each case (a, b, and c) above? Provide an explanation for your answer for each case.

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