(Answered) Suppose someone was attempting to identify proteins in a

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Suppose someone was attempting to identify proteins in a mixture using a polyacrylamide gel electrophoresis, but was struggling to reproduce their results. What is the most likely cause for this?

There are too many charged R-groups on the proteins.
The proteins are too different in size.
The proteins are too similar in size.
SDS was not added to the protein sample.

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