Snouters are small critters that live on both sides of the Ohio River. A population on the north side of the Ohio River is fixed for an allele for blue feet. The population on the south side of the river is fixed for an allele for pink feet. Twenty blue-footed snouters from the north and 80 pink-footed snouters from the south colonize a newly formed island in the middle of the river. Please calculate the following.

A) What are the genotype frequencies at the time of colonization (Parental Generation, P1)? (Just the colonizers before reproduction)

B) What are the allele frequencies at the time of colonization (P1)? (Just the colonizers before reproduction, N = 100)

C) What are the genotype frequencies of the offspring of the next generation (First generation of offspring, F1 ), assuming random mating?

D) What are the allele frequencies of the offspring of the next generation (F1 )?

Heterozygotes have purple feet that are susceptible to snouter purple-foot fungus that sterilizes purple-footed snouters (They cannot reproduce). Assume that the F1 generation above are the parents for the next set of questions.

1) Calculate the allele frequencies of the parents that can breed (F1 with no purple footed individuals). Calculate the genotype and allele frequencies of the offspring (F2) in the second generation assuming random mating (Hint: The sum of the allele frequencies represented by the homozygotes are equal to 1, keep this in mind when you calculate the allele frequency of F1 as parents. It may help if you assume a population size for F1 prior to reproduction.)

2) Has evolution occurred? Why? If the purple foot fungus were to continue on this island for several generations, what color feet would most likely become fixed on the island and why?