bcarrol4 posted a problemTwo membrane preparations have been derived from the plasma membrane of erythrocytes and are present in a buffer solution: (1) right-side-out membrane vesicles, in which the exoplasmic leaflet of the vesicle faces the buffer (equivalent in orientation to that of the exoplasmic leaflet of the intact plasma membrane); and (2) inside-out membrane vesicles in which the exoplasmic leaflet faces the interior of the vesicles.
a. Annexin V is a protein that binds to phosphatidylserine, one of the phospholipids present in cell membranes. Fluorescently labeled annexin V is mixed with each vesicle preparation. The vesicles are washed to remove unbound annexin V and then examined by fluorescence microscopy. Presence of annexin V is detected by green fluorescence, as shown above. What can be deduced from these data?