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Question 1. Chemicals V, W, X, Y, and Z are at separate steps of metabolic pathway as per figure 4.4, p65; great reading. Strains 1, 2, 3 and 4 of Neurospora each have a different mutant gene that renders a given strain incapable of completing one of the chemical reactions in the pathway. Several types of minimal media are prepared; each type supplemented separately with one of the chemicals. "+" = completes reaction & grows. "-" = unable to complete reaction & dies.

What is the second chemical in the pathway?

what is the third chemical in the pathway?

Mutant 1 blocks the step that converts chemical ____ into chemical ____.

Mutant 3 blocks the step that converts chemical ____ into chemical ____.

What chemical is expected to accumulate in mutant strain 4?

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