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Q1. Transformation of competent bacteria, selecting for eg Ampicillin-resistance 4. R Select tet AmpS tetS-E. B. Gene specific identification Colony hybridization. Cleavage of DNA with restriction enzymes provides landmarks and sequence information. How frequently are specific sites found? How many sites are expected? Does this change if the DNA is circular?

Q2. Why would stroke volume increase with an increase in the sympathetic nervous system or an increase in calcium? Why would stroke volume increase when heart rate slows down? If stroke volume is 75 ml/beat and heart rate is 80 beats/min, how many of soda bottles would equal the correct volume (from the quiz)?

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