Q a standard procedure in purifying tubulin is to extract, Ask an Expert

Academics

Q. A standard procedure in purifying tubulin is to extract tissue on ice, after that add GTP and warm the tissue extract to 37°C. The warmed extract is next centrifuged at a speed insufficient to pellet average sized proteins, but enough to pellet large protein complexes. After centrifugation, the supernatant is discarded, and the pellet is redissolved in cold buffer. GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at similar speed described above is again performed. This process is repeated several times.

How will this procedure result in purification of tubulin?

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M9722133

Have any Question?Write your Review or question?

Related Questions in Biology

Igfbp2 rbp4 and factor d post bariatric surgeryigfbp2 what

IGFBP2/ RBP4 and Factor D Post Bariatric Surgery IGFBP2 ( what the normal physiological action in the body? And how it affectedby obesity? andpost bariatric surgery?) RBP4 (what the normal physiological action in the bod ...

Question dietary recommendation and analysis projectin this

Question: Dietary recommendation and analysis project In this project, you will use software to analyze our own diet and make dietary recommendation. You will need submit a report. You can choose any of software if you f ...

Why chemically is nitrogen not more available to living

Why, chemically, is nitrogen not more available to living things? I am looking for the simple chemical reason here

The human microbiome and its relationship to human health

The human microbiome and its relationship to human health, what aspect to the human microbiome is vital?

Assignment 1 create at least a 350-word blog post in

Assignment 1: Create at least a 350-word blog post in Microsoft® Word in response to the following question: Female copperhead snakes have the ability to reproduce both sexually and asexually. In your opinion, which meth ...

If the atomic number of an element is 12 and the atomic

If the atomic number of an element is 12 and the atomic mass is 25. How many protons are there in the nucleus? How many neutrons are there in the nucleus? How many electrons are in the atom?

Question all cancers are characterized by uncontrollable

Question: All cancers are characterized by uncontrollable cell growth and division, and when untreated, may be fatal. Cancers that occur in childhood are usually more malignant than those developed later in life. 1) Why ...

A cross between a person with straight hair and a person

A cross between a person with straight hair and a person with curly hair will produce a child with wavy hair. If 2 people with wavy hair had a child, what are the odds that the child would have straight hair?

Lab assignment -for this assignment you need to document

Lab Assignment - For this assignment you need to document the anatomy you see in the grass frog dissection. USE THE NAME SECTION TASEW GIRMA. Experiment 1: Owl Pellet Dissection 1) Include a photograph to accompany your ...

Two true-breeding stocks of pea plants are crossed one

Two true-breeding stocks of pea plants are crossed. One parents has red, axial flowers and the other has white terminal flowers; all F1 individuals have red, axial flowers. The genes for flower color and location assort ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert