Q 1 What is meant by a haemagglutination inhibition assay?
Q 2 Explain the basis for the negative (saline) control
Q 3 Secretion is the dominant phenotype; Se the dominant allele. Using the gene symbols Se and se write down the genotypes that correspond to the secretor and non-secretor phenotypes
Q 4 If two heterozygous individuals mate, what proportion of the offspring would be expected to be non-secretors? Show your working.
Q 5 Explain the basis for the positive saliva control
Q 6 Prior to the assay both the Ulex reagent and the αA and αB antibodies must be titrated to determine the optimum dilution to use in the assay. Explain why this step is necessary. (Hint. Think what would happen if there was too little or too much Ab added in the assay.)