Problem:

You want to express the anti­bacterial protein Human \betadefensin­3 (HBD3) in the milk of transgenic goats.

Question 1: How would you ensure that the protein was produced in milk and not other tissues?

Question 2: What method would you use to create the transgenic animals? Justify your answer?

Question 3: You generate a number of animals but notice that levels of HBD3 protein vary between individuals. Suggest an explanation for this observation.

Question 4: Why may the activity of the HBD3 protein produced in transgenic goats differ from the human­derived version?

Please describe all the answers of the above questions.