Problemyou are studying a new protein the molecular weight, Ask an Expert

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Problem:

You are studying a new protein. The molecular weight of this protein as determined by gel filtration (size exclusion chromatography) is 350kD. When the protein is subjected to SDS-PAGE, there are 3 bands with molecular masses of 200, 125, and 25. When the protein is subjected to SDS-PAGE in the presence of 2-mercaptoethanol (2-ME), there are 3 bands with molecular masses of 100, 125, and 25.

Required:

Question: Describe the quaternary structure of the full protein, the number of subunits in the protein, the subunit molecular weight, and any interactions/forces that exist between the subunits.

Does anybody know any source of this kind?

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Biology, Academics

Category:- Biology
Reference No.:- M91163296

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