Problemwere trying to do emulsion pcr using ha-coated, Ask an Expert

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Problem:

We're trying to do emulsion PCR using HA-coated polystyrene beads and we're noticing that the beads are seeing drastic issues with thermal degradation above 90C. As PCR has an unfortunate requirement of requiring a high temperature, I was curious about what options are out there to reduce the temperature during PCR denaturation or to stabilize the beads.

Please explain something about it.

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Biology, Academics

Category:- Biology
Reference No.:- M91148879

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