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We are running a gel on the products of a restriction digest to determine the size of an insert. We know the vector backbone size should be ~2.9kb; however, the standard appears to be running too quickly (or our samples too slowly), causing the backbone size to be about 4kb (which distorts our results for the insert size).

Question: What could possibly cause this? Please explain.

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Category:- Biology
Reference No.:- M91149043

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