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Usually DNA sample manipulation is realized with an ice box at hand in order to avoid degradation, and also its storage is set at -20ºC. Nevertheless, DNA has been obtained from really ancient samples that were not in a cold environment and even our DNA is at 36ºC. Furthermore, when performing PCR, samples are put into a massive heat shock and at high temperatures.

Required:

Question 1: How needed are those protocols in order to grant correct DNA quality?

Question 2: How much can a sample be left without need to worry?

Question 3: How does this degradation happen (DNAse free environment)

Does anybody know any source of this kind?

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Biology, Academics

Category:- Biology
Reference No.:- M91163729

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