Problem:

Using Ellman's reagent, find the number of cysteines in a protein that has a concentration of 30 mg/ml solution with a molecular weight of 15,000 Da. Data-- 0.1 ml of the protein was reacted with the reagent and diluted to 10 ml and the absorbance at 412 nm was 1.4 A. Hint, first find the moles of protein used for the experiment,

Question: calculate the moles of Ellman's reagent that reacted. Please show your all work.