Problem:

Transformation & Blue-White Selection

Question 1: What would happen to the bacterial cells if we forgot to add X-gal to our plates? What would happen if we forgot to add IPTG to our plates?

Question 2: What would happen if we inserted the jellyfish GFP protein into the lacZ gene in the same reading frame as the b-gal enzyme, so that when the cell starts to translate the b-gal enzyme, it actually translates the GFP protein? What would we have to add to the agar plates in order to see colonies that fluoresce green?

Please Explain don't just write the answer.