Problemthe genome of virus sv40 is a circular double, Ask an Expert

Academics

Problem:

The genome of virus SV40 is a circular double? Stranded DNA molecule 5 kb long (i.e., it contains 10,000 bases).

a. A 1 kb region is amplified by PCR for four cycles. (Assume that specific amplification of the target region occurs.) What fraction of the total DNA does the target sequence constitute? Show your work.

b. After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N. After five generations, what is the ratio of light: heavy DNA strands? Show your work.

Please describe the work done for the mentioned problem.

View complete question

Biology, Academics

Category:- Biology
Reference No.:- M91145997

Have any Question?Write your Review or question?

Related Questions in Biology

You take a small section of leaf and view it underneath a

You take a small section of leaf and view it underneath a microscope. You count 25 stomata. Now you want to calculate how many stomata would be in a leaf the size of a penny. a) Calculate the area of the view of view (A= ...

The ch50 assay is a traditional clinical assay that

The CH50 assay is a traditional clinical assay that measures the function of complement components. In a CH50 assay patient blood is drawn and serum separated from it (serum has no cells). Patient serum is mixed with ant ...

What is the complementary strand for 5-atgcatgcatgccc-3how

What is the complementary strand for: 5'-ATGCATGCATGCCC-3' How many turn(s) will this strand have? Are Eukaryotic cells always diploid during S phase whereas bacteria are only haploid at the end of DNA replication?

You have been allocated a cdna accession number from the

You have been allocated a cDNA accession number from the NCB' database (see table below on page 2). Your task is to design experimental strategies that will allow you to: clone the cDNA in different expression vectors us ...

How does enzymes lower activation energy when they catalyze

How does enzymes lower activation energy when they catalyze reactions?

Krebs cycletca cyclewhat are the reactantswhat are the

Krebs Cycle/TCA cycle What are the reactants? What are the products? What is the net ATP gain? Where does it take place in prokaryotes vs eukaryotes?

Question darwin was not the first to consider evolution as

Question: Darwin was not the first to consider evolution as a process but he did come up with the first effective explanation for how it happens. Describe Darwin's theory of evolution by natural selection. Explain how th ...

Why chemically is nitrogen not more available to living

Why, chemically, is nitrogen not more available to living things? I am looking for the simple chemical reason here

In summer squash white fruit w is dominant over yellow

In summer squash, white fruit (W) is dominant over yellow fruit (w) and the "disk" fruit shape (D) is dominant over "sphere" shape (d). Determine the genotypes of the parents in the following cases: A. White disk crossed ...

Soap and detergent molecules have a long hydrophobic tail

Soap and detergent molecules have a long, hydrophobic tail and a polar, hydrophilic head. They are sometimes referred to as bridge molecules because they allow oils and fats to be suspended and dissolved in water (whic ...

4,153,160 Questions Asked
13,132 Experts
2,558,936 Questions Answered

Ask Experts for help!!

Looking for Assignment Help?

Start excelling in your Courses, Get help with Assignment

Write us your full requirement for evaluation and you will receive response within 20 minutes turnaround time.

Ask Now Help with Problems, Get a Best Answer

Recent Questions

Ask Biology Expert