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Question: Which of the following is a way in which the mechanism by which topoisomerase I breaks a DNA strand differs from the mechanism of phosphodiester bond formation? Can be multiple answer.

a) Only one involves an OH group acting as a nucleophile

b) Only one involves the use of ATP

c) Only one involves a nucleotide-amino acid bond

d) Only one involves the release of a pyrophosphate molecule

e) Only one requires DNA Polymerase

Give reasoning with your answer.

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